Review



cxcr4  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Miltenyi Biotec cxcr4
    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, <t>CXCR4,</t> Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Cxcr4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cxcr4/product/Miltenyi Biotec
    Average 94 stars, based on 15 article reviews
    cxcr4 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT"

    Article Title: c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

    Journal: Molecular Cancer

    doi: 10.1186/s12943-025-02486-5

    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Figure Legend Snippet: A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Techniques Used: Microscopy, Clinical Proteomics, Expressing, Flow Cytometry, Injection, Comparison, Phospho-proteomics, Isolation



    Similar Products

    cxcr4  (Miltenyi Biotec)
    94
    Miltenyi Biotec cxcr4
    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, <t>CXCR4,</t> Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Cxcr4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cxcr4/product/Miltenyi Biotec
    Average 94 stars, based on 1 article reviews
    cxcr4 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265  (Bioss)
    93
    Bioss phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265
    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, <t>CXCR4,</t> Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Phycoerythrin Pe Conjugated Mouse Anti Rat Cxcr4 Antibodies 265, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265/product/Bioss
    Average 93 stars, based on 1 article reviews
    phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    anti cxcr4 mouse monoclonal polyclonal antibody  (Proteintech)
    96
    Proteintech anti cxcr4 mouse monoclonal polyclonal antibody
    CXCL12 and its <t>receptor</t> <t>CXCR4</t> were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).
    Anti Cxcr4 Mouse Monoclonal Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cxcr4 mouse monoclonal polyclonal antibody/product/Proteintech
    Average 96 stars, based on 1 article reviews
    anti cxcr4 mouse monoclonal polyclonal antibody - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    anti human cxcr4 antibody  (Miltenyi Biotec)
    96
    Miltenyi Biotec anti human cxcr4 antibody
    CXCL12 and its <t>receptor</t> <t>CXCR4</t> were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).
    Anti Human Cxcr4 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cxcr4 antibody/product/Miltenyi Biotec
    Average 96 stars, based on 1 article reviews
    anti human cxcr4 antibody - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    mouse igg2a chb6  (Bio-Rad)
    93
    Bio-Rad mouse igg2a chb6
    CXCL12 and its <t>receptor</t> <t>CXCR4</t> were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).
    Mouse Igg2a Chb6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg2a chb6/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    mouse igg2a chb6 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    cxcr4  (Bio-Rad)
    93
    Bio-Rad cxcr4
    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are <t>CXCR4+.</t> Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.
    Cxcr4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cxcr4/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    cxcr4 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    9d9  (Bio-Rad)
    93
    Bio-Rad 9d9
    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are <t>CXCR4+.</t> Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.
    9d9, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9d9/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    9d9 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    156 gd conjugated mouse anti human cxcr4  (fluidigm)
    93
    fluidigm 156 gd conjugated mouse anti human cxcr4
    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are <t>CXCR4+.</t> Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.
    156 Gd Conjugated Mouse Anti Human Cxcr4, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/156 gd conjugated mouse anti human cxcr4/product/fluidigm
    Average 93 stars, based on 1 article reviews
    156 gd conjugated mouse anti human cxcr4 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    3156029b 158 gd conjugated mouse anti human cd10 fluidigm  (fluidigm)
    93
    fluidigm 3156029b 158 gd conjugated mouse anti human cd10 fluidigm
    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are <t>CXCR4+.</t> Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.
    3156029b 158 Gd Conjugated Mouse Anti Human Cd10 Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3156029b 158 gd conjugated mouse anti human cd10 fluidigm/product/fluidigm
    Average 93 stars, based on 1 article reviews
    3156029b 158 gd conjugated mouse anti human cd10 fluidigm - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    3155008b 156 gd conjugated mouse anti human cxcr4 fluidigm  (fluidigm)
    93
    fluidigm 3155008b 156 gd conjugated mouse anti human cxcr4 fluidigm
    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are <t>CXCR4+.</t> Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.
    3155008b 156 Gd Conjugated Mouse Anti Human Cxcr4 Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3155008b 156 gd conjugated mouse anti human cxcr4 fluidigm/product/fluidigm
    Average 93 stars, based on 1 article reviews
    3155008b 156 gd conjugated mouse anti human cxcr4 fluidigm - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Journal: Molecular Cancer

    Article Title: c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

    doi: 10.1186/s12943-025-02486-5

    Figure Lengend Snippet: A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Article Snippet: Cxcr4 , 130-118−682 , Miltenyi , PE.

    Techniques: Microscopy, Clinical Proteomics, Expressing, Flow Cytometry, Injection, Comparison, Phospho-proteomics, Isolation

    CXCL12 and its receptor CXCR4 were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).

    Journal: Animal Bioscience

    Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

    doi: 10.5713/ab.24.0640

    Figure Lengend Snippet: CXCL12 and its receptor CXCR4 were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).

    Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

    Techniques: Binding Assay, Immunofluorescence, Staining, Over Expression, Expressing, Phospho-proteomics

    CXCL12 targets the receptor CXCR4 to activate the JAK/STAT signaling pathway and regulate the expression of related genes.

    Journal: Animal Bioscience

    Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

    doi: 10.5713/ab.24.0640

    Figure Lengend Snippet: CXCL12 targets the receptor CXCR4 to activate the JAK/STAT signaling pathway and regulate the expression of related genes.

    Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

    Techniques: Expressing

    Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are CXCR4+. Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.

    Journal: Frontiers in Immunology

    Article Title: Tenascin-C regulates CXCR4 + B cell migration and cortex formation in the developing bursa of Fabricius

    doi: 10.3389/fimmu.2025.1636140

    Figure Lengend Snippet: Structure of lymphoid follicles in the chicken bursa of Fabricius. (A) Haematoxylin-eosin stained paraffin-embedded section of an 8-week-old chicken bursa of Fabricius. Each bursal follicle contains a central medullary and an outer cortical region, separated by a basement membrane (dashed line). (B) Electron micrograph of the cortex (see the outlined area in A) shows several reticular cells (arrowheads) scattered among cortical B cells. The outlined area marks the cortico-medullary epithelium. (C) The euchromatic nuclear structure of reticular cells (orange) differs from the heterochromatic nuclear structure of lymphocytes. (D) Reticular cells of the medulla are of epithelial origin and express cytokeratin intermediate filaments. In contrast, reticular cells of the cortex are of mesenchymal origin and are desmin+. (E, E’, E’’) The dotted line indicates the cortico-medullary border; the dashed line marks the outer border of the cortex. Desmin+ mesenchymal reticular cells co-express vimentin intermediate filaments. (F) Desmin+/vimentin+ reticular cells in the cortex uniformly express the CXCL12 chemokine ligand shown by in situ hybridization. Magnified view in the inset. Arrowheads show CXCL12+/vimentin+ cortical reticular cells. (G) B cells within the cortex are CXCR4+. Laminin marks the basement membrane under the cortico-medullary border and around cortical capillaries.

    Article Snippet: CXCR4 , 9D9 , Dr. Sonja Hartle, BioRad (MCA6012GA) , mouse IgG 2A.

    Techniques: Staining, Membrane, In Situ Hybridization

    Tenascin-C and CXCR4 show a complementary expression pattern in the follicular cortex. (A, B) Double immunostaining of tenascin-C and CXCR4 in the adult bursal follicle shows robust tenascin-C expression in CXCR4 low/dim regions along the cortico-medullary border (dashed line). Tenascin-C immunoreactivity is highest around cortical capillaries (inset) at the E-cadherin+ cortico-medullary epithelium (B) and gradually decreases between the capillaries (double arrows) and towards outer cortical regions. (C-E) EdU incorporation reveals that CXCR4 high B cells within the outer region of the cortex are highly proliferative. Laminin expression marks the basement membrane along the cortico-medullary border and around cortical capillaries.

    Journal: Frontiers in Immunology

    Article Title: Tenascin-C regulates CXCR4 + B cell migration and cortex formation in the developing bursa of Fabricius

    doi: 10.3389/fimmu.2025.1636140

    Figure Lengend Snippet: Tenascin-C and CXCR4 show a complementary expression pattern in the follicular cortex. (A, B) Double immunostaining of tenascin-C and CXCR4 in the adult bursal follicle shows robust tenascin-C expression in CXCR4 low/dim regions along the cortico-medullary border (dashed line). Tenascin-C immunoreactivity is highest around cortical capillaries (inset) at the E-cadherin+ cortico-medullary epithelium (B) and gradually decreases between the capillaries (double arrows) and towards outer cortical regions. (C-E) EdU incorporation reveals that CXCR4 high B cells within the outer region of the cortex are highly proliferative. Laminin expression marks the basement membrane along the cortico-medullary border and around cortical capillaries.

    Article Snippet: CXCR4 , 9D9 , Dr. Sonja Hartle, BioRad (MCA6012GA) , mouse IgG 2A.

    Techniques: Expressing, Double Immunostaining, Membrane

    Effect of tenascin-C and tenascin-C bound CXCL12 on B cell migration and adhesion. (A) Schematic illustration of embryonic BF organ culture experiments. (B) E13 chicken bursal folds containing CXCR4+ B cells were used to study cell migration induced by the CXCL12 chemokine. (C) CXCR4+ bursal cells show limited migration on untreated plastic cell culture dish surface in response to 100 ng/ml CXCL12. The inset shows a magnified view of CXCR4+ cells (red). DAPI (blue) was used for nucleus staining. (D) Coating the plastic surface with 20 µg/ml fibronectin induces robust CXCR4+ cell migration from the explant. Note the presence of extensive microvilli and filopodia on the surface of a CXCR4+ cell (inset). (E) In contrast, the presence of tenascin-C markedly inhibits CXCL12-induced cell migration, the CXCR4+ cells were spherical (inset). (F) Quantitative analysis of the migration distance of CXCR4+ cells for each experimental group. Data presented with the standard error of the mean. (G) CXCR4+/chB6+ B cells isolated from E18 BF and cultured on the surface of CXCL12 immobilized to tenascin-C induced polarization of B cells with long filopodia, (H) which is effectively blocked by treatment with the CXCR4 receptor antagonist AMD3100, where B cells were spherical. (I) Quantitative analysis of cell morphology of bursal B cells for each experimental group. The length of filopodia of chB6+ cells of 20 different high-power (200×) fields was counted in untreated (RMPI-1640 culture medium) and AMD3100-treated cultures. chB6+ cells with filopodia longer than the cell diameter were scored. These are the representative images of the morphologic phenotypes detected in 3 separate experiments, including screening of at least 200 cells in each experiment. Significance levels: **p<0.01, ***p<0.001, ns, not significant. Error bars represent the standard error of the mean.

    Journal: Frontiers in Immunology

    Article Title: Tenascin-C regulates CXCR4 + B cell migration and cortex formation in the developing bursa of Fabricius

    doi: 10.3389/fimmu.2025.1636140

    Figure Lengend Snippet: Effect of tenascin-C and tenascin-C bound CXCL12 on B cell migration and adhesion. (A) Schematic illustration of embryonic BF organ culture experiments. (B) E13 chicken bursal folds containing CXCR4+ B cells were used to study cell migration induced by the CXCL12 chemokine. (C) CXCR4+ bursal cells show limited migration on untreated plastic cell culture dish surface in response to 100 ng/ml CXCL12. The inset shows a magnified view of CXCR4+ cells (red). DAPI (blue) was used for nucleus staining. (D) Coating the plastic surface with 20 µg/ml fibronectin induces robust CXCR4+ cell migration from the explant. Note the presence of extensive microvilli and filopodia on the surface of a CXCR4+ cell (inset). (E) In contrast, the presence of tenascin-C markedly inhibits CXCL12-induced cell migration, the CXCR4+ cells were spherical (inset). (F) Quantitative analysis of the migration distance of CXCR4+ cells for each experimental group. Data presented with the standard error of the mean. (G) CXCR4+/chB6+ B cells isolated from E18 BF and cultured on the surface of CXCL12 immobilized to tenascin-C induced polarization of B cells with long filopodia, (H) which is effectively blocked by treatment with the CXCR4 receptor antagonist AMD3100, where B cells were spherical. (I) Quantitative analysis of cell morphology of bursal B cells for each experimental group. The length of filopodia of chB6+ cells of 20 different high-power (200×) fields was counted in untreated (RMPI-1640 culture medium) and AMD3100-treated cultures. chB6+ cells with filopodia longer than the cell diameter were scored. These are the representative images of the morphologic phenotypes detected in 3 separate experiments, including screening of at least 200 cells in each experiment. Significance levels: **p<0.01, ***p<0.001, ns, not significant. Error bars represent the standard error of the mean.

    Article Snippet: CXCR4 , 9D9 , Dr. Sonja Hartle, BioRad (MCA6012GA) , mouse IgG 2A.

    Techniques: Migration, Organ Culture, Cell Culture, Staining, Isolation

    Schematic illustration of B cell migration in the cortex of bursal follicles. 1) CXCR4 high medullary B cells migrate towards high concentrations of CXCL12 in the cortex, along tenascin-C free zones between cortical capillaries. CXCL12-abundant reticular (CAR) cells within the cortex establish the high local chemokine concentrations. Soluble CXCL12 interacts with extracellular tenascin-C, forming CXCL12-rich zones around cortical capillaries. 2) Expansion of CXCR4 high B cells occurs in outer cortical regions, and CXCL12-rich zones around cortical capillaries prevent premature B cell emigration towards extra-bursal sites. 3) Bursal B cell emigration is dependent on the downregulation of the CXCR4 receptor, allowing cell migration through CXCL12-rich inner cortical zones. CXCR4 low/dim B cells reach the periphery through cortical capillaries.

    Journal: Frontiers in Immunology

    Article Title: Tenascin-C regulates CXCR4 + B cell migration and cortex formation in the developing bursa of Fabricius

    doi: 10.3389/fimmu.2025.1636140

    Figure Lengend Snippet: Schematic illustration of B cell migration in the cortex of bursal follicles. 1) CXCR4 high medullary B cells migrate towards high concentrations of CXCL12 in the cortex, along tenascin-C free zones between cortical capillaries. CXCL12-abundant reticular (CAR) cells within the cortex establish the high local chemokine concentrations. Soluble CXCL12 interacts with extracellular tenascin-C, forming CXCL12-rich zones around cortical capillaries. 2) Expansion of CXCR4 high B cells occurs in outer cortical regions, and CXCL12-rich zones around cortical capillaries prevent premature B cell emigration towards extra-bursal sites. 3) Bursal B cell emigration is dependent on the downregulation of the CXCR4 receptor, allowing cell migration through CXCL12-rich inner cortical zones. CXCR4 low/dim B cells reach the periphery through cortical capillaries.

    Article Snippet: CXCR4 , 9D9 , Dr. Sonja Hartle, BioRad (MCA6012GA) , mouse IgG 2A.

    Techniques: Migration