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cxcr4  (Miltenyi Biotec)


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    Miltenyi Biotec cxcr4
    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, <t>CXCR4,</t> Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Cxcr4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+cxcr4/CD184+(CXCR4)+Antibody%2C+anti-mouse%2C+REAfinity/pmc12849452-37-0-4
    Average 93 stars, based on 18 article reviews
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    Images

    1) Product Images from "c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT"

    Article Title: c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

    Journal: Molecular Cancer

    doi: 10.1186/s12943-025-02486-5

    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)
    Figure Legend Snippet: A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Techniques Used: Microscopy, Clinical Proteomics, Expressing, Flow Cytometry, Injection, Comparison, Phospho-proteomics, Isolation

    Related Articles

    Isolation:

    Article Title: Engineering a heterogeneous tissue from pluripotent stem cells
    Article Snippet: Single cell suspensions were generated by incubating hiPSC colonies for five minutes in Accutase (Stem Cell Technologies, Vancouver) at 37° C., subsequently resuspending this single cell solution in 5 ml of DMEM/F-12 medium containing 10 μM Y-27632 dihydrochloride (Tocris Biosciences, UK) solubilized in cell culture grade DMSO (Sigma-Aldrich), centrifuging it at 500 rpm for 3 min and respending the pellet in mTeSR-1 or DMEM/F-12 supplemented with Y-27632 at a final concentration of 10 μM for counting. .. Magnetic Isolation of Cells CXCR4 and CD34 isolations were performed at day 5 and 10 respectively using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (Miltenyi). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Article Title: Engineering a heterogeneous tissue from pluripotent stem cells
    Article Snippet: Single cell suspensions were generated by incubating hiPSC colonies for five minutes in Accutase (Stem Cell Technologies, Vancouver) at 37° C., subsequently resuspending this single cell solution in 5 ml of DMEM/F-12 medium containing 10 μM Y-27632 dihydrochloride (Tocris Biosciences, UK) solubilized in cell culture grade DMSO (Sigma-Aldrich), centrifuging it at 500 rpm for 3 min and respending the pellet in mTeSR-1 or DMEM/F-12 supplemented with Y-27632 at a final concentration of 10 μM for counting. .. Magnetic Isolation of Cells CXCR4 and CD34 isolations were performed at day 5 and 10 respectively using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (Miltenyi). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Magnetic Beads:

    Article Title: Engineering a heterogeneous tissue from pluripotent stem cells
    Article Snippet: Single cell suspensions were generated by incubating hiPSC colonies for five minutes in Accutase (Stem Cell Technologies, Vancouver) at 37° C., subsequently resuspending this single cell solution in 5 ml of DMEM/F-12 medium containing 10 μM Y-27632 dihydrochloride (Tocris Biosciences, UK) solubilized in cell culture grade DMSO (Sigma-Aldrich), centrifuging it at 500 rpm for 3 min and respending the pellet in mTeSR-1 or DMEM/F-12 supplemented with Y-27632 at a final concentration of 10 μM for counting. .. Magnetic Isolation of Cells CXCR4 and CD34 isolations were performed at day 5 and 10 respectively using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (Miltenyi). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Article Title: Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
    Article Snippet: Collected media were assayed for AAT (Genway Biotech), fibrinogen (Genway Biotech) Oncostatin M (Abcam) and albumin (Bethyl Labs) using commercially available ELISA kits. .. CXCR4 and CD34 isolations were performed at days 5 and 10, respectively, using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (MACS beads, Miltenyi Biotech). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Article Title: Engineering a heterogeneous tissue from pluripotent stem cells
    Article Snippet: Single cell suspensions were generated by incubating hiPSC colonies for five minutes in Accutase (Stem Cell Technologies, Vancouver) at 37° C., subsequently resuspending this single cell solution in 5 ml of DMEM/F-12 medium containing 10 μM Y-27632 dihydrochloride (Tocris Biosciences, UK) solubilized in cell culture grade DMSO (Sigma-Aldrich), centrifuging it at 500 rpm for 3 min and respending the pellet in mTeSR-1 or DMEM/F-12 supplemented with Y-27632 at a final concentration of 10 μM for counting. .. Magnetic Isolation of Cells CXCR4 and CD34 isolations were performed at day 5 and 10 respectively using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (Miltenyi). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Magnetic Cell Separation:

    Article Title: Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
    Article Snippet: Collected media were assayed for AAT (Genway Biotech), fibrinogen (Genway Biotech) Oncostatin M (Abcam) and albumin (Bethyl Labs) using commercially available ELISA kits. .. CXCR4 and CD34 isolations were performed at days 5 and 10, respectively, using mouse anti-CXCR4 and mouse anti-CD34 antibodies conjugated to magnetic beads (MACS beads, Miltenyi Biotech). .. The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    other:

    Article Title: Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
    Article Snippet: The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.

    Article Title: Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6
    Article Snippet: The suspensions were then flowed through Miltenyi magnetic bead LS separation columns alongside a magnet to trap the positive cell population.



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    Mouse Miltenyi Biotec 130 092 575 Percp Cytm5 5 Anti Mouse Cxcr4 Biolegend 146507 Pe Anti Mouse Cd86 Biolegend 105007, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Journal: Molecular Cancer

    Article Title: c-Rel drives pancreatic cancer metastasis through fibronectin-integrin signaling-induced isolation stress resistance and EMT

    doi: 10.1186/s12943-025-02486-5

    Figure Lengend Snippet: A Colony-forming frequency was assessed by the ability of cells to form an organoid in Matrigel (one-way ANOVA, p=0.0015). Tukey's multiple comparisons test results are shown in the figure. B Representative microscopy images of spheroids formed under nonadherent conditions. The average spheroid diameter was quantified and analyzed (genotype factor p=0.0227). C Representative spheroid images and their diameter quantification. The spheroid medium was supplemented with either human plasma fibronectin (pFN1) or cellular fibronectin with EDB domain + type II domains 8−14 (EDB cFN1.3) (treatment factor p= n.s.) D Surface expression of selected proteins on spheroids as assessed by flow cytometry analysis (one-way ANOVA test for CD61 p=0.0244, CD133, EpCAM, CD44, CXCR4, Sca1 p= n.s.). Tukey's multiple comparisons test results are shown in the figure. E Representative macroscopic and microscopic images of the transplanted mouse lungs. The number of cell lines used per genotype are CKP=5, GCKP=5 and FNGCKP=4. Each cell line was injected two times to two individual mice. Nested one-way ANOVA was used for both analyses. Tukey's multiple comparisons test results are shown in the figure. Unless otherwise indicated, for all analyses, two-way ANOVA was used. ANOVA p values are given in the figure or their legends. Šídák's multiple comparison tests between genotypes are shown in the figures. F Schematic depicting the function of c-Rel in PDAC pathophysiology. c-Rel is an oncogenic factor involved in PDAC survival and metastasis. Increased c-Rel expression remodels the ECM by converting the collagen-rich stroma to a fibronectin-rich stroma. Cancer cells undergo epithelial-to-mesenchymal transition, increasing EMP by reducing the surface expression of E-cadherin and contractility via Src and Stat3 phosphorylation. Once isolation stress is induced under nonadherent conditions, c-Rel induces a niche enriched with fibronectin, coupled with increased Itgb3, Itga5 and Itgav expression. These changes are also supported by EMP, where c-Rel directly induces EMT-TFs such as Snai1, Snai2, Zeb2, and Tgfb . Increased tolerance to isolation stress ultimately drives metastasis. Created in BioRender. Kabacaoglu, D. (2025)

    Article Snippet: Cxcr4 , 130-118−682 , Miltenyi , PE.

    Techniques: Microscopy, Clinical Proteomics, Expressing, Flow Cytometry, Injection, Comparison, Phospho-proteomics, Isolation

    apMNKQ2 targets the CSC compartment in PDAC. A, B Left: Representative images of colonies ( A ) or spheres ( B ) in control- or apMNKQ2-transfected Panc354 cells. Right: Mean ± SEM in the crystal violet optical density (OD) ( A ) or number (no.) of spheres/ml ( B ) in control- or apMNKQ2-transfected Panc215 or Panc354 cells. * p < 0.05, **** p < 0.0001; as determined by one-sample t test. C Mean ± SEM of the percentage of CD24-, CD133-, CXCR4- or ALDH-positive cells in control- or apMNKQ2-transfected cells 24 h post transfection. *** p < 0.001, **** p < 0.0001; as determined by one-sample t test. D Schematic of the transfection groups, apMNKQ2-FITC transfection efficiency determined by flow cytometry and subsequent dilution of cells for ELDA determination. E Percentage of tumor take (number of tumors confirmed/number of injections) for subcutaneously injected control (CTL)- and apMNKQ2 (Q2)-transfected cells was determined over the course of 124 days. F Tumor weight (g) o tumors extracted at indicated times in ( E ). ns = not significant; nd = not determined. G CSC frequencies determined using the extreme limiting dilution analysis algorithm ( http://bioinf.wehi.edu.au/software/elda/index.html ) (left, 95% CI) and images of resected tumors for the dilution 5 × 10 5 (right)

    Journal: Journal of Biomedical Science

    Article Title: Aptamer-based inhibition of MNK1 reduces pancreatic ductal adenocarcinoma growth by targeting cancer stem cells

    doi: 10.1186/s12929-026-01275-6

    Figure Lengend Snippet: apMNKQ2 targets the CSC compartment in PDAC. A, B Left: Representative images of colonies ( A ) or spheres ( B ) in control- or apMNKQ2-transfected Panc354 cells. Right: Mean ± SEM in the crystal violet optical density (OD) ( A ) or number (no.) of spheres/ml ( B ) in control- or apMNKQ2-transfected Panc215 or Panc354 cells. * p < 0.05, **** p < 0.0001; as determined by one-sample t test. C Mean ± SEM of the percentage of CD24-, CD133-, CXCR4- or ALDH-positive cells in control- or apMNKQ2-transfected cells 24 h post transfection. *** p < 0.001, **** p < 0.0001; as determined by one-sample t test. D Schematic of the transfection groups, apMNKQ2-FITC transfection efficiency determined by flow cytometry and subsequent dilution of cells for ELDA determination. E Percentage of tumor take (number of tumors confirmed/number of injections) for subcutaneously injected control (CTL)- and apMNKQ2 (Q2)-transfected cells was determined over the course of 124 days. F Tumor weight (g) o tumors extracted at indicated times in ( E ). ns = not significant; nd = not determined. G CSC frequencies determined using the extreme limiting dilution analysis algorithm ( http://bioinf.wehi.edu.au/software/elda/index.html ) (left, 95% CI) and images of resected tumors for the dilution 5 × 10 5 (right)

    Article Snippet: For the detection of CD24, CD133 or CXCR4 cell surface marker expression, cells were incubated with a 1:5 dilution of a mouse anti-human CD24 PE (BD Cat no. 555428), a 1:50 dilution of a mouse anti-human CD133/1 Vio Bright R667 Ab (Miltenyi Cat no. 130–111–756) or a 1:50 dilution of a mouse anti-human CXCR4 (CD184) PE Ab (Miltenyi Cat no. 130–117–354).

    Techniques: Control, Transfection, Flow Cytometry, Injection, Software

    CXCL12 and its receptor CXCR4 were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).

    Journal: Animal Bioscience

    Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

    doi: 10.5713/ab.24.0640

    Figure Lengend Snippet: CXCL12 and its receptor CXCR4 were expressed and localized in the ovary, with their binding activating the JAK/STAT signaling pathway. (A) Immunofluorescence staining of ovarian follicles revealed that CXCL12 was co-localized with its receptor CXCR4 in ovarian follicles. (B) CXCL12 overexpression significantly promoted the expression of CXCR4 , JAK2 and STAT1 . (C) Knocking down CXCL12 significantly inhibited the expression of CXCR4 , JAK2 and STAT1 . (D) CXCL12 overexpression in GCs promoted the protein expression of CXCR4, JAK2 and STAT1 as well as the phosphorylation of JAK2 and STAT1. When CXCL12 was knocked down, the results were reversed. (E) MSX-122 inhibitor-based treatment further confirmed that CXCL12 promoted the total protein and phosphorylation levels of JAK2 and STAT1 (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001).

    Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

    Techniques: Binding Assay, Immunofluorescence, Staining, Over Expression, Expressing, Phospho-proteomics

    CXCL12 targets the receptor CXCR4 to activate the JAK/STAT signaling pathway and regulate the expression of related genes.

    Journal: Animal Bioscience

    Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

    doi: 10.5713/ab.24.0640

    Figure Lengend Snippet: CXCL12 targets the receptor CXCR4 to activate the JAK/STAT signaling pathway and regulate the expression of related genes.

    Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

    Techniques: Expressing